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gene exp cfb hs00156060 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp cfb hs00156060 m1
    Gene Exp Cfb Hs00156060 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfb/Gene+Exp%2E+CFB%2C+Hs00156060_m1/us12595477-1467-25--1
    Average 89 stars, based on 1 article reviews
    gene exp cfb hs00156060 m1 - by Bioz Stars, 2026-10
    89/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Beta-cell-specific C3 deficiency exacerbates metabolic dysregulation and insulin resistance in obesity.
    Article Snippet: RT-qPCR was performed on cDNA using TaqMan Gene Expression Master Mix 169 (ThermoFisher Scientific, #4369016), and Gene Expression Assays following the 170 Jo urn al Pr e-p roo f 8 manufacturer’s instructions. .. The following Gene Expression Assays were used: Complement 171 factor D (Cfd) Mm01143935_g1; Complement factor B (Cfb) Mm00433909_m1; C3 172 Mn01232779_m1; Cd163 Mm00474091_m1; Adgre1 (F4/80) Mm00802529_m1; Ccl2 (Mcp-173 1) Mm00441242_m1; Itgam (CD11b) Mm00434455_m1; Il1 Mm00434228_m1; Tnfa 174 Mm00443258_m1; Il6 Mm00446190_m1; Reference genes were Hprt Mm01545399_m1 and 175 B2m Mm00437762_m1. ..

    Article Title: Beta-cell-specific C3 deficiency exacerbates metabolic dysregulation and insulin resistance in obesity
    Article Snippet: RT-qPCR was performed on cDNA using TaqMan Gene Expression Master Mix (ThermoFisher Scientific, #4369016), and Gene Expression Assays following the manufacturer’s instructions. .. The following Gene Expression Assays were used: Complement factor D ( Cfd ) Mm01143935_g1; Complement factor B ( Cfb ) Mm00433909_m1; C3 Mn01232779_m1; Cd163 Mm00474091_m1; Adgre1 (F4/80) Mm00802529_m1; Ccl2 (Mcp-1) Mm00441242_m1; Itgam (CD11b) Mm00434455_m1; Il1β Mm00434228_m1; Tnfa Mm00443258_m1; Il6 Mm00446190_m1; Reference genes were Hprt Mm01545399_m1 and B2m Mm00437762_m1. ..

    Article Title:
    Article Snippet: Briefly, cDNA was synthesized from total RNA (Trizol, Thermo-Fisher) using random hexamers, and reverse transcriptase (Thermo-Fisher). .. Real-time quantitative PCR was performed using Assay-on Demand Gene Expression Products (Thermo-Fisher) specific for: C1qL1 (Rn01773625_m1), C3 (Rn00566466_m1), C5 (Rn01436156_m1), C6 (Rn00598444_m1), C7 (Rn01402708_m1), C8g (Rn01438689_g1), CFB (Rn01526084_g1), CFD (Rn01535436_g1) and CD59 (Rn00563923_m1). .. GAPDH (Rn01775763_g1) was used as housekeeping endogenous gene.

    other:

    Article Title: Complement Factor B-modulating compositions and methods of use thereof
    Article Snippet: 2 μl of cell lysate was added to the reaction mixture containing 5 μl TaqMan 1-Step qRT-PCR Mix, 1 μl CFB(FAM) TaqMan Gene Expression Assay (Hs00156060_m1 or Mm00433909_m1), 1 μl GAPDH(VIC) TaqMan Gene Expression Assay (Hs02786624_g1 or Mm99999915_g1) and 11 μl RT-PCR grade nuclease-free water in a MicroAmp Optical 96-well plate (0.2 mL). qPCR was performed using a QuantStudio3 qPCR machine with the following cycles: 50° C. for 1 minute, 95° C. for 20 seconds and 40 cycles at 95° C. for 15 seconds and 60° C. for 1 minute.

    Article Title: Divergent responses to SARS-CoV-2 infection in bronchial epithelium with pre-existing respiratory diseases.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Human Airway epithelial cells MucilAirTM Male, 27years, Non-smoker, CF, CFTR F508del homozygous Epithelix CF-MD43702 Human Airway epithelial cells MucilAirTM Female, 30years, Non-smoker, CF, CFTR F508del homozygous Epithelix CF-MD67901 Human Airway epithelial cells MucilAirTM Female, 33years, Non-smoker, CF, CFTR F508del homozygous Epithelix CF-MD051902 Human Airway epithelial cells MucilAirTM Male, 22years, Non-smoker, CF, CFTR F508del homozygous Epithelix CF-MD063701 Human Airway epithelial cells MucilAirTM Female, 31yearears, Smoker (<1 pack per day (PPD) x 17years), COPD Epithelix MD068301 Human Airway epithelial cells MucilAirTM Female, 53years, Smoker (3 PPD x 30 years), COPD Epithelix MD047201 Human Airway epithelial cells MucilAirTM Male, 58years, Smoker (1/2 PPD x 40 years), COPD Epithelix MD068901 Human Airway epithelial cells MucilAirTM Male, 60years, Smoker (1 PPD x 45 years), COPD Epithelix MD077701 TMPRSS2 activity Human Airway epithelial cells MucilAirTM Male, African American, 17years, No Smoker, No Pathology reported Epithelix MD0889 Human Airway epithelial cells MucilAirTM Female, African American, 54years, No smoker, No Pathology reported Epithelix MD0868 Human Airway epithelial cells MucilAirTM Female, 21yearears, No Smoker, CF, CFTR F508del homozygous Epithelix MD0607 Human Airway epithelial cells MucilAirTM Male, Caucasian, 58years, Smoker (2 PPD x 22 years), COPD Epithelix MD0903 Oligonucleotides Primers for nsp14: HKU-ORF1b-nsp14F: 50-TGGGGYTTTACRGGTAACCT-30; reverse primer HKU-ORF1b-nsp14R: 50-AACRCGCTTAACAAAGCACTC-30; probe HKU-ORF1b-nsp141P: 50-FAM-TAGTTGTGATGCWATCATG ACTAG-TAMRA-30 Eurogentech GAPDH Taqman gene expression assay Applied Biosystems Hs02786624_g1 C3 Taqman gene expression assay Applied Biosystems Hs00163811_m1 CFB Taqman gene expression assay Applied Biosystems Hs00156060_m1 Software and algorithms AOZAN software ENS Paris STAR algorithm https://github.com/alexdobin/ STAR/releases Version 2.5.2b (Continued on next page) iScience 28, 111999, March 21, 2025 e2

    Real-time Polymerase Chain Reaction:

    Article Title:
    Article Snippet: Briefly, cDNA was synthesized from total RNA (Trizol, Thermo-Fisher) using random hexamers, and reverse transcriptase (Thermo-Fisher). .. Real-time quantitative PCR was performed using Assay-on Demand Gene Expression Products (Thermo-Fisher) specific for: C1qL1 (Rn01773625_m1), C3 (Rn00566466_m1), C5 (Rn01436156_m1), C6 (Rn00598444_m1), C7 (Rn01402708_m1), C8g (Rn01438689_g1), CFB (Rn01526084_g1), CFD (Rn01535436_g1) and CD59 (Rn00563923_m1). .. GAPDH (Rn01775763_g1) was used as housekeeping endogenous gene.

    Article Title: NNMT inhibition in cancer-associated fibroblasts restores antitumour immunity.
    Article Snippet: Reverse transcription of 2 μg total RNA was performed using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, 4374966). .. Quantitative PCR (qPCR) was conducted with FAM-labelled TaqMan probes targeting mouse complement factor C3 (Thermo Fisher Scientific, Mm01232779_m1) and mouse complement factor B (Thermo Fisher Scientific, Mm00433909_m1). .. Reactions were run using TaqMan Fast Advanced Master Mix (Bio-Rad, 1725134) on the CFX Opus 96 Real-Time PCR System (Bio-Rad, 12011319) and analysed using the 2 C−∆∆ t method.



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